Abstract: Aiming at the problem of strand breakage caused by aeolian vibration at suspension clamps of conductors, this study investigates the local dynamic bending strain characteristics and fatigue life. Typical wind speed segments are extracted based on the Weibull distribution of regional meteorological data. An ANSYS model of the LGJ-400/35 conductor is established, incorporating equivalent bending stiffness considering interlayer slip, and dynamic responses are obtained through graded dynamic simulations. The dominant frequencies for each wind speed segment are calculated using the Strouhal formula, and fatigue assessment is conducted with the rainflow counting method and Miner"s linear cumulative theory. The results show that the dominant frequencies of aeolian vibration at the clamp range from 6 to 28 Hz, with a total annual number of vibration cycles of 3.86×10? and an annual cumulative damage degree of 0.0612. After applying a safety margin reduction using the comprehensive correction coefficient specified in theSOverhead Transmission Line Design Code, the predicted actual fatigue life is approximately 11.4 years. The study confirms the long-term cumulative damage mechanism of aeolian vibration characterized by high frequency and low amplitude, and identifies that the combined resonance induced by low-to-moderate wind speeds of 2–3 m/s (dominant frequencies of 13–20 Hz) is the most critical factor leading to fatigue failure, accounting for 82.5% of the cumulative damage. This provides a quantitative basis for targeted frequency-band anti-vibration design of transmission lines.